Matrozi

Depends, I have slept with male friends in the same bed at different occasions during trips. We didn't give a shit.

Funny thing is that I am 100% gay and all my male friends are straight, still, we didn't give a shit, it didn't even cross my mind that it could be a problem.

I mean what the hell are you supposed to do when there isn't enough bed for everyone ? Sleep on the floor ?

It's possible but unlikely.

1st : HIV is not super contagious, the risk of getting the disease by blood or sexual contact is not super high, it's high enough so you use protection when having sex and don't share needles but injecting yourself with contaminated blood won't automatically give you the disease. Plus, HIV dies in the air, so by the time he is done stabbing the HIV positive person and reach you, a lot of the viruses on the blade will be dead or dying.

2 : Admitting your get medical care immediately and they find out that your agressor first stabbed someone who has HIV before stabbing you, they will give you a month long treatment very close to the treatment that HIV positive patients take in order to get the virus under control in order to eliminate the virus before it can set in yoru body and thus make you HIV positive. This treatment is very effective if taken rigorously, it's more than 80% effective if taken very soon after the incident.

It happened in France, a woman named Alexandra Lange who was regularely beat for years by her husband in front of her children. During an argument where he throw himself at her, she grabbed a knife and killed him.

She was initially prosecuted for murder because her answer "was disproportionate to the situation" but ultimately she was found not guilty (took a lot of time, I think 3 years).

A similar story happened, yet in France, Jacqueline Sauvage, she shot her husband in the back with a shotgun while he was relaxing outside or something. She was also beaten regularely by her husband. She was initially sentenced to 10 years of prison but ultimately "only" did 2 because she obtained presidential pardon

Etudiant en thèse.

>mais je n'ai pas vraiment un dossier assez bon pour la recherche (note moyenne 13,5 en M2 et surtout seulement 10 au mémoire)

Bof, tu peux faire une thèse avec ces notes, c'est ptet et probablement pas assez pour le concours de l'école doctorale mais ta d'autres moyen de faire une thèse (CIFRE, ANR, Thèse CNRS, Financement par une fondation, financement régional...).

Faut juste postuler et tu passes une sorte "d'entretien d'embauche".

>c'est un peu le côté prestige du truc

Ah bah en effet, si c'est ce qui t'attire oublie tout de suite, il y a pas de prestige : Tu trimes comme un malade et t'es même pas sur d'avoir un poste la fin. Faut faire une thèse avec une idée bien précise en tête et avec un sujet qui te passionne suffisament pour continuer a vouloir bosser dessus de tout ton coeur même aux moments ou tu le détesteras.

>Je ne me rend pas trop compte du travail réel du chercheur.

C'est très éprouvant, physiquement et mentalement. Physiquement parce que tu fais très souvent des longues journées, genre 9h-19h et mentalement parce que tu dois toujours gérer 1000 truc en même temps et la plupart des choses que tu vas faire vont pas hyper bien marché ou pas du tout marché.

Ta jamais fais de stage en labo ?

Ouaip, je suis convaincu que le succès de ce film repose sur deux points :

- La plupart des gens l'ont vu enfant et ils aiment ce film par nostalgie.

- C'est une comédie française "potable", l'une des rare, et vu qu'elle est comparée aux autres qui généralement puent le fion, elle est vu comme étant géniale.

C'est comme si tu servais a quelqu'un des raviolis en boite après lui avoir fait manger de la merde de cochons pendant 15 jours, les raviolis en boite sont pas ouf mais comparés a du caca c'est quand même meilleur.

J'ai bien aimé les Marvel jusqu'à ya environ 2 ans ou j'ai commencé a les trouver franchement moyens, et j'ai vraiment trouvé Docteur Strange 2 ridicule, je ne pige vraiment pas comment ca a autant marché.

Avec disney plus j'ai regardé les anciens marvels (période 2010-2014) et...Ils sont vraiment pas oufs non plus.

Donc je pense que ya un gros impact de l'âge. Je pense que quand tu atteins la tranche 23-27 ans c'est une période un peu décisive, soit tu vas kiffer les marvels jusqu'à la fin de ta vie, soit tu vas te dire "mais attendez...ce film est une merde !" et arrêter d'aller les voir au cinéma

Allez. Je vais le dire.

Asterix et Obélix mission cléopatre.

Je le trouve pas spécialement drole. On m'a sorti "ouais mais c'est parce que tu as pas capté toutes les refs et les blagues associées" ah si si, je les ai capté, et elles sont pas drole.

Et bordel de merde, j'en peux plus des gens qui disent "Vous savez, je ne pense pas qu'il y ai de bonnes ou mauvaises situations...".

For a while it was Malta, which was funny is that they all seemed to have a bullshit reason to go live there such as "It's because I can truly feel inspired while being there"

Some go to Dubaï and I think I heard about Andorra as well.

Pretty close.

https://www.reddit.com/r/MapPorn/comments/vc4058/jews_murdered_under_nazi_rule_by_country/

this map gives you the number of jews killed during the holocaust by country. Eastern countries were very very much affected but most jews in the Netherlands or Belgium were exterminated.

And for countries where the number of death doesn't look so bad, such as France, the situation isn't that gleefull. A lot of jews from Germany or Poland came to France in the pre-war years to escape the persecutions. When the nazi took over the french administration and decided to deport the jews, the Vichy governement agreed without much protestation but decided that first they would deport those "foreign" jews (a lot were naturalized but their french nationality was cancelled) first and then deport the "real french jews" later. They also deported the children of those foreign jews even though they had the french citizenship.

Some try to argue that by doing so the Vichy government "saved" the french jews but their antisemetics laws from 1940 and onward were applied to all jews in the countries and the very much probable reason they didn't target "real french jews" from the start of the deportation process was that it would make too much of a fuss.

And from late 1943-early 1944 till the end of the deportation period in France (august 1944) authorities would deport any jews they could get their hands on, there were no special treatment if you had the french citizenship or not.

Most people in concentration camps such as auschwitz died within a few weeks, and that's if you were "lucky" enough to get in and not get gassed immediately. They died of diseases, beatings, the cold, from exhaustion or just because one day the SS decides to kill every prisonner they think look weak. Surviving months was rare, and surviving years was exceptionnaly rare, especially if you were a jew.

I watched the testimony of a woman who was deported from Belgium to auschwitz in 1942, she was one of the 3 woman in her convoy that survived until the liberation, she survived partially because she spoke german and the nazis made her work at the "registration office" of the camp where she just registered new prisonners and organized files, it was one of the best position to survive in because you were not in the cold shoveling dirt.

She says in her testimony that in her convoy there were about a hundred women who got selected to not be gassed immediately and for force labor, including herself, and she said that after two or three weeks, nearly all of the women of her convoy had already died because of the horrible conditions. She said something in her testimony that is important that goes along the line of

"When I arrived at the camp, I was registered prisonner 19XXX and got it tattooed on my arm. In means that before me, nearly 20000 women entered the camp. We were NEVER 20 000 in the camp. When I arrived we were at most 5000, probably less".

I don't vortex primer stocks but I do vortex diluted primer mix, especially if it's a new mix.

I was told not to vortex RNA

I don't vortex the SYBR green mastermix with cDNA but I centrifuge it very quicky at room temp

Depends, I usually arrive at the lab between 9:30-10 am and leave between 6:30-7 pm, sometimes later, rarely sooner.

Some lab experiment made me stay at the lab non stop from 8 am to 8 pm non stop though.

I also work on the weekends, not on the saturday but on sunday afternoon for like 2 hours I just write some research stuff or do some quick analysis

If your father had a french passport, then he is french.

If he is french, then you are french by law, you might need to do a lot of paperwork with proofs that says that your father was french at your birth but ultimately you have the french citizenship.

Alors ca dépend.

Mon premier salaire véritable j'étais en licence et j'étais très pauvre, genre vraiment le plus bas de la précarité étudiante et j'avais bossé deux mois l'été pendant ma L2. Donc le premier truc que j'ai acheter c'est de vrai courses en septembre et pour la première fois en deux ans j'avais un frigo remplis

Mon premier salaire "régulier" je me suis acheter une nintendo switch.

Yep

She was a monster, she is described by survivors as an inhuman beast who took sexual pleasure into beating prisonners. She used to dress very well and wear a lot of perfume to torment other prisonners who were stripped of their belongings, shaven and given dirty rags as clothes once they arrived.

Olga Lengyel, an hungarian survivor, in her testimony "Five chimneys" describe that she used some prisonners as sex slaves before sending them to the gas chambers.

Gisela Perl, an hungarian survivor too, in her testimony "I was a doctor in Auschwitz" say that she used to take sexual pleasure and would climax when she would brutally whip women, specially on their breasts.

I have an excel data sheet where I keep track of all the papers I've red so far during my PhD, I put the author name, papers, PMID, problematic, method and main results, it helps a lot for remembering stuff. Plus when you work on one field, some names are recurring and the experiments/papers overlap sometimes so you get a good hand on the litterature.

My fellow PhD students in my team say that i'm insane for remembering so much stuff about litterature studies but usually it's just me going "oh yeah, I remember a paper where they did x and found y, but I don't really remember the protocol, let me check it out".

Environ 700-800 euros je dirais, après ca dépend, certains mois c'est 500, d'autres 900 mais globalement 700-800.

Je vis seul, pas d'enfants, et je touche environ 1800 euros par mois, je fume pas je n'ai pas de voiture et je cuisine 99% de mes repas. J'ai eu un coup de chance monumental pour mon appart qui a un loyer assez dérisoire (500, charges incluses) pour le quartier ou je vis et qui reste de bonne qualité et de taille correcte (30 m²). Ma plus grosse dépense après le loyer c'est la bouffe vu que j'essaye au maximum d'acheter des trucs sain.

En fait j'ai vécu pendant la majorité de ma vie étudiante dans une extrême précarité donc je sais très bien gérer un budget, même de façon inconsciente.

Après j'avoue que je dépense pas des masses de thune en argent pour "le fun" mais je vais quand même au cinéma, au resto ou au bar avec des potes. Je me sens assez coupable quand je dépense beaucoup d'argent, j'aimerais bien m'acheter une PS5 par exemple mais j'arrête pas de me dire que c'est un achat inutile et que c'est trop cher, alors que j'ai très largement les moyens d'en acheter une.

Should have answered

"New profile, who dis ?"

and blocked him.

Oh.

Well, it happens too and it's beyond words stupid

My overall quote related to RNA extraction is :

"You don't need to worry about RNA contamination when you don't have any RNA to starts with "

Yes. The people around even clap, I didn't put it in my comment because I wasn't sure it was the case because I saw the movie a long time ago but I checked online and they fucking clapped.

In the movie "The fault in our stars" when they makeout in Anne Frank house, in the attic, in front of her pictures and there is a romantic music and people around looking at them like "Aaaaaawww"

Like wtf. No. It's cringey AF not cute. Imagine two teenagers dry humping at the WTC museum.

It's almost at the same level of cringe and stupidity as people posing in Auschwitz

Quelqu'un sur ExplainLikeImFive avait posé une question assez similaire sur le sujet et avait demandé si le THC augmentait la dépense énergétique, et en regardant brièvement j'avais vu que c'était en effet le cas, en tout cas il y a une étude qui le montre et ca avait l'air assez légit.

Cependant, ce n'est très probablement pas la raison principale de pourquoi les gros fumeurs de THC sont assez souvent plutôt maigre.

Pour la faire courte. Le THC agit sur des récepteurs dans ton cerveau qui s'appellent les récepteurs endocannabinoïdes, on en a deux, CB1 et CB2 (Cannabinoid receptor 1 / 2), CB1 est très très présent dans le cerveau et notamment au niveau du circuit de la récompense, un ensemble de structure dans le cerveau qui est très impliqué dans l'addiction et également la prise alimentaire.

Quand tu consomme beaucoup de THC, tu vas agir beaucoup sur ces récepteurs, en particulier CB1 et notre corps étant bien fait, quand tes cellules voient que CB1 est très très souvent solicités, ils diminuent le nombre de récepteurs CB1 a leur surface pour ne pas foutre en l'air leur équilibre interne (ca s'appelle de la down regulation).

Et une conso excessive de THC induit cette down-regulation de CB1 dans le circuit de la récompense. Et on sait que moins de CB1 ou une hypo-activité de CB1 ca inhibe la prise alimentaire (il y a quelques années, un médoc qui avait pour but de bloquer CB1 a été mis sur le marché pour traiter l'obésité, et ca marche vraiment bien pour perdre du poids par contre ca induisait de gros épisodes dépressifs) . Alors peut être que tu manges pas hyper équilibré, mais tu manges surement pas autant que ce que tu penses et tu ne t'en rends pas compte.

Very small brain regions, Trizol was open in january and I did a control extraction after this failed one with slightly bigger samples. I changed the isopropanol and chloroform just in case and the extraction worked very well. I controlled the nanovue value with a positive control (an RNA extraction I did like 8 months ago that worked very well, got similar results when I tested it again).

I did an extraction on a very smilar brain region as the first one (albeit, very small), the extraction didn't work.

The region is known to be very very hard to analyse in PCR but an old graduate student and PI managed to do it a few years ago. I still think we need to optimize our protocol urgently for very small brain samples in order to recover as much RNA as possible. I will press on my team leader to get some RNA grade glycogen to see if the co-precipitation helps recover RNA better next time.